Special Seminar of Environmental Analysis, "The preparation method of standard solution, and the operation method of GC/MS and LC/MS/MS"
Date: May 12, 2009
Venue: Graduate School at Shenzhen, Tsinghua University, China
Kyoto University Global COE Program "Global Center for Education and Research on Human Security Engineering for Asian Megacities"
Kyoto University EML Program "international Center for Human Resource Development in Environmental Management"
Number of attendants: 20
The Shenzhen base has various analytical instruments such as GC/MS, ICP, and an atomic absorption spectrophotometer. Up to now, we have held seminars for the purpose of attaining knowledge of environmental analysis. When putting analyses into practice, however, there is still the need for a lot of know-how and technology, and there has been no lecture that fulfilled the need. For this reason, to carry out accurate environmental analysis, it is necessary to newly give technical guidance for students. This seminar aimed at mastering the preparation of the standard solution for calibration curve, and measuring method and maintenance of GC/MS and LC/MS/MS. Moreover, practical technique instruction on GC/MS, which has been used frequently at Graduate School at Shenzhen, Tsinghua University, was given to the participants, with the cooperation of SHIMADZU TECHNO-RESEARCH. In addition to all the students of Graduate School at Shenzhsen, Tsinghua University, the participants of the seminar included staff from the Shenzhen Water Quality Centre, which is a governmental agency.
In this seminar, practical skill instructions were given using GC/MS to measure triphenyl phosphate and LC/MS/MS to measure ethinyl estradiol. First, we prepared standard solutions for calibration curve using each substance. Then, standard solution of 10 mg/L, 20 mg/L, 50 mg/L, and 100 mg/L were prepared using the reference standard solution of 2 mg/L. The participants got directions on the setting method of the analysis conditions, using GC/MS and LC/MS/MS, for measuring the standard solution prepared by each group. Finally, the participants evaluated the correctness of dilution operation on the linearity of a calibration curve. The participants acquired the proper usage of laboratory glassware and microsyringes through the seminar. Furthermore, they deepened their understanding of the apparatus by having operated GC/MS and LC/MS/MS. At the end of the seminar, each participant received a certificate. An anonymous survey was carried out after the seminar, and many participants answered that the seminar had been beneficial for them. In addition, there was a remark on having the seminar for a longer period of time, indicating a need to continuously hold seminars.